| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pcDNA3.1/V5-His A |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 5502 dp |
| 5' 测序引物及序列: | CMV-F |
| 3' 测序引物及序列: | BGH rev |
| 载体标签: | His Tag (6x), V5 Epitope Tag |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 新霉素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 哺乳动物细胞表达C末端V5和6xHis标记蛋白的载体。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
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pcDNA3.1/V5-His A载体质粒基本信息
pcDNA3.1/V5-His A质粒图谱载体图谱和pcDNA3.1/V5-His A载体序列质粒序列多克隆位点信息
pcDNA3.1/V5-His A质粒载体简介
pcDNA3.1/V5-His A质粒序列载体序列
LOCUS pcDNA3.1-V5-His A 5502 bp ds-DNA circular SYN 24-OCT-2017
DEFINITION Mammalian vector for expressing C-terminally V5- and 6xHis-tagged
proteins. For other reading frames, use pcDNA(TM)3.1/V5-His B or
pcDNA(TM)3.1/V5-His C.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1 V5-His A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5502)
AUTHORS Thermo Fisher (Invitrogen)
TITLE Direct Submission
JOURNAL Exported Saturday, November 16, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT The cloned gene must include a Kozak sequence and start codon.
FEATURES Location/Qualifiers
source 1..5502
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 902..999
/note="MCS"
/note="multiple cloning site"
CDS 1000..1041
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
CDS 1051..1068
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 1097..1321
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1367..1795
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1809..2138
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1989..2124
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2205..2999
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3175..3296
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3747..4335)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4506..5366)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5367..5471)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca
961 gcacagtggc ggccgctcga gtctagaggg cccttcgaag gtaagcctat ccctaaccct
1021 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
1081 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
1141 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
1201 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
1261 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
1321 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
1381 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
1441 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
1501 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
1561 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
1621 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
1681 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
1741 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
1801 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
1861 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
1921 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
1981 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
2041 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
2101 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
2161 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
2221 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
2281 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
2341 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
2401 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
2461 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
2521 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
2581 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
2641 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
2701 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
2761 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
2821 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
2881 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
2941 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
3001 cgggactctg gggttcgcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
3061 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
3121 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
3181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
3241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
3301 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
3361 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
3421 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
3481 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
3541 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
3601 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
3661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
3721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
3781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
3841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
3901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
3961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
4021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
4081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
4141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
4201 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
4261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
4321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
4381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
4441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
4501 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
4561 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
4621 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
4681 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
4741 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
4801 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
4861 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
4921 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
4981 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
5041 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
5101 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
5161 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
5221 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
5281 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
5341 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
5401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
5461 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
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